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43 Found helpful • 5 Pages • Essays / Projects • Year: Pre-2021
1. Did you observe any difference in bacterial growth between protocol 1, 2, and 3? Comment on your class results. Compare these three protocols, and list the major factors that can cause contamination in the aseptic transfer protocol 1, 2, and 3. 2. Comment on class result with respect to differences in filter types, differences in filter assemblies, and overall on the confidence you would have in using this type of sterilisation process in preparation of pharmaceutical products. List the factors that may cause contamination during filtration. 3. Although chlorocresol (a phenolic component) is a strong anti-microbial agent, why in this exercise it did demonstrate the lowest antimicrobial activity? Why did you observe lower CFU reduction when the lotion was inoculated with Saccharomyces cerevisiae rater than Staphylococcus epidermidis. 4. Are the MICs dependent on the micro-organisms chosen for the test? Gram+ or Gram–: which one is more resistant? Why? 5. Comment on the effect of adding EDTA to the antimicrobial agent and suggest a reason for that effect. 6. What is the nature of the “incompatibility” of chlorhexidine with aqueous cream and does this “incompatibility” affect its antimicrobial activity? 7. Why did you first filter the solution through the membrane filter and discard it? 8. Why is steam sterilisation used to sterilise the potassium chloride ampoules?
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