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LDH Report of MBLG1001

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This experiment measures the activity of the enzyme lactate dehydrogenase (LDH). The activity of LDH can be measured by monitoring the increase in

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LDH Report of MBLG1001
Topics this document covers:
Chemical pathology Lactate dehydrogenase Biochemistry
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Topics this document covers:
Chemical pathology Lactate dehydrogenase Biochemistry
Sample Text:
The activity of LDH can be measured by monitoring the increase in absorption at 340nm (340nm E = 6.2 mM-1cm-1) at pH 9.5 in the presences of Hydrazine reacting specifically with ketone groups such as the one in pyruvate can make the rate of NADH production linear with time. Buffer concentration is between 0.1 and 0.32 and kept on ice to prevent denaturing of enzyme. Measurement of reaction was taken over a short period of time, 2 – 3 minutes at room temperature. Tables Normal Serum 100uL 150uL Patient Serum 10uL 20uL ΔA (absorption)= 0.0775 0.16 0.1425 0.216 C (concentration)= 0.0129 mM/min 0.027 mM/min 0.02375 mM/min 0.036 mM/min 0.129 μmol/ml/min 0.18 μmol/ml/min 2.2 μmol/ml/min 1.7 μmol/ml/min Table 1. Expression of activity in normal serum and patient serum. Elevation fold Normal Activity 0.1 U/ml Patient Activity 1.84...
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