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Investigation on Enzyme Activity and Kinetics
CHE 506 - Reaction Engineering Laboratory
2 Found helpful • 9 Pages • Essays / Projects • Year Uploaded: 2021
The main objective of the experiment is to investigate the effect of pH and temperatures on enzyme activity and study the relationship between substrate concentration and the maximum velocity of an enzyme. The method of determining the Michaelis Constant, Km used in the experiment is by Hanes - Woolf Method. After sitting in a specific temperature water bath for a period of time, the amylase solution is mixed with the tapioca starch solution. The hydrolysis process is then allowed to take place. The spectrophotometer is used to determine the absorbance value. The optimum condition for the amylase enzyme is at substrate concentration, [S] = 2 %. The Michaelis constant obtained by using the Hanes - Woolf Method is Km = 101.6746 min-1. The plotted graph of absorbance values versus parameters has been accomplished. The standard curve for concentration versus absorbance had also been plotted for glucose solution. The study of the optimum conditions for the bio reaction is critical in order to obtain the maximum output of product and to determine the limit of the enzyme
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