In any aerobic fermentation, the dissolved oxygen concentration becomes an important control variable. It is crucial to have a solid understanding of how oxygen is transferred to cells in a reactor. The main objective of the experiment is to determine the Mass Transfer Coefficient (k L a) of a bioreactor using distilled water as the simulating media. The method used for this experiment is the static gassing out method. The materials used are distilled water and nitrogen gas. The apparatus are bioreactor, stopwatch, and air compressor. 3 parameters need to be considered in this experiment which are agitation, aeration, and temperature. For agitation parameter The volumetric mass transfer of coefficient for temperature 27°C, 30°C and 40°C at 1 VVM 0.0001s-1 , 0.0002s-1 , 0.0003 s-1 , 0.0003s-1 , and 0.0003s-1 , respectively. Meanwhile, for 2VVM the value of k L A is 0.0004s-1 , 0.0005s-1 , 0.0005s-1 , 0.0007s-1 , and 0.0009s-1 respectively for each of the agitation. Next, for agitation the volumetric mass transfer of coefficient for temperature 27°C, 30°C and 40°C at 1 VVM 0.0001s-1 , 0.0002s-1 , 0.0003 s-1 , 0.0003s-1 , and 0.0003s-1 , respectively. Meanwhile, for 2VVM the value of k L A is 0.0004s-1 , 0.0005s-1 , 0.0005s-1 , 0.0007s-1 , and 0.0009s-1 respectively for each of the agitation. The last parameter is aeration at 1VVM, and 2VVM for 27°C, 30°C, and 40°C. The volumetric mass transfer of coefficient at 100 RPM, 200 RPM, 300 RPM, 400 RPM, and 500 RPM is tabulated in table 1. All these 3 parameters show that if the agitation speed, aeration rate, and temperature increase, the volumetric mass transfer will increase too. This experiment has met the objectives successfully.