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Guinea pig ileum consists of various receptors, including histamine and muscarinic receptors. The drugs’ effects on these receptors and the relationship of agonism and antagonism can be determined by examining the contractile response of guinea pig ileum in response to the drug added. An agonist binds and interacts with the receptor and producing the effects, whereas antagonist interacts with the receptor, but blocks the response from the receptor. Histamine acts as a neurotransmitter that binds to histamine receptors and causing contractions of smooth muscles in the ileum.

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