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The muscarinic and histamine receptors are among the several receptors which can be found in the guinea pig ileum. Muscarinic receptors are either ligand-gated G protein coupled receptors or are metabotropic. These receptors are involved in the parasympathetic nervous system and it has 7 transmembrane α-helices coupled to intracellular effector system such as activation or inhibition of protein kinase for a specific response via a G-protein which acts as a secondary messenger. M1, M2, M3, M4, and M5 are the 5 subtypes of M receptor and different subtypes of G protein, including Gs, Gq, Go, and Gi protein, are coupled to each M receptor

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