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DNA Isolation Report

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Completed DNA Isolation report with all the answers to the questions and table. Mark: Credit

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4 Pages Essays / Projects 1-2 Years old
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DNA Isolation Report
Topics this document covers:
Biology Molecular biology Biochemistry Chemistry Polymerase chain reaction Genetics Laboratory techniques Electrophoresis Gel electrophoresis DNA Nucleic acid DNA extraction
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Topics this document covers:
Biology Molecular biology Biochemistry Chemistry Polymerase chain reaction Genetics Laboratory techniques Electrophoresis Gel electrophoresis DNA Nucleic acid DNA extraction
Sample Text:
Chloroform was handled in fume hood under the supervision of demonstrator. Sealed test tube was used. RESULTS AND ANALYSIS Volume in TE(mL) 2 Dilution 20 A 260 1.097 A 280 0.531 A 260 :A 280 2.07 Diluted [DNA] (μg/mL) 54.85 Original [DNA] (mg/mL) 1.097 Yield (mg) 2.194 Yield (mg/g cells) 4.388 COMMENT ON YOUR RESULTS Comment: The value of A 260 :A 280 ratio of the result is 2.07, which is higher than the theoretical value. The experimental yield (4.39 mg/g cells) is higher than theorectical yield (5.04 mg/g cells). Having seen the results of the gel electrophoresis run with your DNA that was treated with RNase what more can you discern about the purity of your DNA The DNA is not pure, it is...
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