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Scientific Report on Beta-galactosidase Practical

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In this experiment, the concentration of β-galactosidase is determined in E. coli using Isopropyl β-D-1-thiogalactopyranoside (IPTG), which inhibits the lac repressor. The time of induction of lac operon was determined in the process. An investigation is also done to analyze the outcome of the addition of various agents, antibiotics and glucose solutions during the induction of β-galactosidase in E. coli.

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Scientific Report on Beta-galactosidase Practical
Topics this document covers:
Gene expression Biology Biochemistry Molecular biology Galactosides Lac operon Isopropyl β-D-1-thiogalactopyranoside Ortho-Nitrophenyl-β-galactoside Beta-galactosidase Inducer Allolactose Catabolite repression
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Topics this document covers:
Gene expression Biology Biochemistry Molecular biology Galactosides Lac operon Isopropyl β-D-1-thiogalactopyranoside Ortho-Nitrophenyl-β-galactoside Beta-galactosidase Inducer Allolactose Catabolite repression
Sample Text:
The lac operon consists of genes LacA, LacZ and LacY that synthesises beta- galactosidase to break down lactose to glucose and galactose. Beta-galactosidase is also responsible for the synthesis of allolactose, which is the natural inducer of the lac operon. There are a few key components present in the lac operon. The regulatory genes produced repressors that bind to the operator site of the lac operon. This prevents mRNA from binding to the promoter next to the operator site due to steric hindrance. As a result, mRNA will not be able to transcribed the 3 structural genes and metabolism of lactose will not be able to occur. When glucose level is high, the concentration of cyclic adenosine monophosphate (cAMP) is low because the presence of glucose dephosphorylates EIIA, which deactivate Adenyl cyclase and...
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