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Plant Biology Lab Report

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To investigate the role of the CYCB1 gene and CYCB1 protein throughout the cell cycle and its response to auxin in the plant species Arabidopsis thaliana.

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11 Pages Essays / Projects < 1 Year old
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Plant Biology Lab Report
Topics this document covers:
Molecular biology Biology Nature Chemistry Auxin Real-time polymerase chain reaction Arabidopsis thaliana Gene expression Reverse transcription polymerase chain reaction GUS reporter system Reporter gene
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Topics this document covers:
Molecular biology Biology Nature Chemistry Auxin Real-time polymerase chain reaction Arabidopsis thaliana Gene expression Reverse transcription polymerase chain reaction GUS reporter system Reporter gene
Sample Text:
Abstract The CYCB1 gene is a cell cycle regulatory gene that is linked with mitotic activity in the plant shoot and root apical meristems. This study investigates the CYCB1 gene and CYCB1 protein in the plant cell cycle and in response to auxin. A dot blot assay was performed to measure the abundance of the CYCB1 protein throughout the cell cycle in synchronised Arabidopsis thaliana cell cultures. The results of the dot blot assay indicate that the CYCB1 protein has a maximal accumulation at 9 hours post aphidicolin treatment (PAT) which corresponds with the mitotic index. GUS staining was performed to analyse the spatial expression of the CYCB1 gene in a transgenic Arabidopsis thaliana CYCB1 promoter-reporter gene (GUS) and its response to auxin. The results from the GUS staining showed that the CYCB1 gene was upregulat...
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